Rhythmicity in Nitrate Reductase Activity in Wheat Embryos during Germination
نویسندگان
چکیده
Nitrate reductase (NR) activity, induced by germination in KNO3 showed a strong rhythmicity during the early stages of germination of wheat embryos. Peaks of activity occurred approximateiy every 12 h. Tbere was no relationship hetween the amounts of nitrate in tbe embryos and enzyme activity. Tbe fluctuations in enzyme activity did not appear to be due to reversible inactivation of the enzyme. Some evidence suggests that tbe fluctuations in enzyme activity may result from a more rapid degradation of enzyme during the decreasing phase of the rhythm.
منابع مشابه
Use of doubled haploid technology for development of stable drought tolerant bread wheat (Triticum aestivum L.) transgenics.
Anther culture-derived haploid embryos were used as explants for Agrobacterium-mediated genetic transformation of bread wheat (Triticum aestivum L. cv CPAN1676) using barley HVA1 gene for drought tolerance. Regenerated plantlets were checked for transgene integration in T₀ generation, and positive transgenic haploid plants were doubled by colchicine treatment. Stable transgenic doubled haploid ...
متن کاملاثر نیترات پتاسیم بر فعالیت آنزیمهای آنتیاکسیدان در مرحله جوانهزنی بذور زوال یافته ماریتیغال
Seed aging increases activity of reactive oxygen molecules and antioxidant enzymes have a key role against increased oxidative stress which is caused by free radicals. Effect of seed priming on increasing of these enzymes activity and its impacts on seed germination characteristics of deteriorated milk thistle seeds was studied in University of Mohaghegh Ardabili in 2013. Factoral experiment ba...
متن کاملTHE EFFECT OF SALT STRESS ON MALATE DEHYDROGENASE IN WHEAT
Effect of various NaCI treatments (0, 50, 100, 200 and 300 mM) at different growth and development stages (tillering, boot swollen, flowering and anthesis) of two wheat cultivars on the kinetic activity and PAGE electrophoretic pattern of leaf malate dehydrogenase was studied under greenhouse conditions. Ghods was salt-sensitive and Boolani was salt-tolerant. In general, in response to salinti...
متن کاملDe Novo Biosynthesis of Deoxyribonucleic Acid Polymerase during Wheat Embryo Germination.
An 8-fold enhancement in the activity of a DNA-dependent DNA polymerase was found in extracts from germinating wheat (Triticum vulgare var. Florence) embryos, as compared to the activity found in extracts from ungerminated embryos. The enhancement of this activity during the first hours of germination is concomitant to the increase of a Dnase activity. The two activities could be separated and ...
متن کاملRelationship between Poly(Adenosine 5'-diphosphate-ribose) Synthesis and Transcriptional Activity in Wheat Embryo Chromatin.
Chromatin-bound poly(adenosine 5'-diphosphate [ADP]-ribose) synthetase activity was highest in ungerminated wheat (Triticum aestivum L., cv. Mukakomugi) embryos, and it decreased after germination within 14 hours. In contrast, transcriptional activity was lowest in ungerminated wheat embryos, and it increased during germination for 24 hours or more. Histones, H1, H2A/H2B, basic nonhistone chrom...
متن کامل